Limited resolution, decreased magnification, and a poorer surface view of the specimen are all downsides of using a light microscope. The user of a compound light microscope must adjust to optical inversion, which causes the specimen to appear to be displaced in the opposite direction of movement.
The level of resolution achievable with a light microscope is limited by visible light. Magnification ranges from 500x to 1500x, which is significantly less than electron microscopes, which have magnifications of 160,000x or more. Light microscopes, in general, require thin, tiny, transparent specimens for optimal viewing. The invention of the electron microscope in the 1930s allowed scientists to see minute aspects of cellular structure with more freedom.